A Mathematical Model of Cyclin B1 Dynamics at the Single Cell Level in Osteosarcoma Cells
نویسندگان
چکیده
Cyclin B1 tracking provides information on cell cycle progression and cell-cycle regulator dynamics. We have developed a mathematical model which describes the continuous tracking of cyclin B1 through the cell cycle at the single cell level, including interactions with the cyclin B1 inhibitor, p21. The cell line used is a cancer cell line, human osteosarcoma (U-2 OS). An examination of the sensitivity of the model is presented, where the aim is to identify those parameters which have most influence on the cyclin B1 profile and its changes through the cell cycle. High temporal resolution cyclin B1 data involving non-invasive techniques (green fluorescent protein, GFP) were used to validate the model.
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